请使用支持JavaScript的浏览器!
主营:血浆蛋白
℡ 4000-520-616
℡ 4000-520-616
Haematologic Technologies/Mouse Fibrinogen/MCI-5150/1 mg
产品编号:MCI-5150
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 蛋白类>天然蛋白>球蛋白>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: haemtech
公      司:Haematologic Technologies Inc.
公司分类:
Haematologic Technologies/Mouse Fibrinogen/MCI-5150/1 mg
商品介绍
FormulationLyophilizedfrom20mMNaCit,20mMNaPO4,pH7.3
Storage4°C
Purity>95%bySDS-PAGE
ActivityDeterminationN/A
ShelfLife(properlystored)12months
DomainStructureofFibrinogen
ThetrinodulararrangementoftheAα,Bβ,andγchains,theIIacleavagesites(IIa)releasingFPAandFPB,aswellastheplasmincleavagesites(Pm)yieldingFragmentDandFragmentEareshown.

SampleGelInformation:

GelNovex4-12%Bis-Tris
Load1µgperlane;purifiedhumanfibrinogen
BufferMOPS
StandardSeeBluePlus2;Myosin(191kDa),PhosphorylaseB(97kDa),BSA(64kDa),GlutamicDehydrogenase(51kDa),AlcoholDehydrogenase(39kDa),CarbonicAnhydrase(28kDa),MyoglobinRed(19kDa),Lysozyme(14kDa)

Overview:

Thethrombin(IIa)catalyzedcleavageofsolublefibrinogen(Fbg)toformfibrin(Fbn)istheterminalproteolyticeventinthecoagulationcascade.ThesesolubleFbnmonomersspontaneouslypolymerizetoformaninsolubleFbnnetworkwhichisstABIlizedbythefactorXIIIacatalyzedcrosslinkingoflysandgluresiduesofαandγchains.ThisFbnnetworkisthemajorproteincomponentofthehemostaticplug.

Plasmafibrinogenislargeglycoprotein(Mr=340,000)synthesizedintheliverandcirculatingataconcentrationof2.6mg/ml.Itisadisulfidelinkeddimercomposedof3pairsofdisulfidelinkednon-identicalpolypeptidechains(Aα,Bβandγ).NotablefeaturesoftheAαchainaretheN-terminalpeptide(fibrinopeptideA(FPA,1-16)),factorXIIIacrosslinkingsitesand2phosphorylationsites.Whensynthesized,Fbgisfullyphosphorylated,butcirculatesatonly20-30%phosphorylation.TheBβchaincontainsfibrinopeptideB(FPB,1-14),oneofthe3N-linkedcarbohydratemoieties(Mr=2500)andanN-terminalpyroglutamicacid.TheγchaincontainstheotherN-linkedglycosylationsiteandafactorXIIIacrosslinkingsites.The2elongatedsubunits((AαBβγ)2)arealignedinanantiparallelmannerformingatrinodulararrangementofthesixchains.Thenodesareformedbydisulfideringsbetweenthe3parallelchains.Thecentralnode(n-disulfideknot,Edomain)isformedbytheN-terminiofallsixchainsheldtogetherby11disulfidebonds.Thisregioncontainsthe2IIa-sensitivesites.ThereleaseofFPAbycleavageatR16-G17generatesFbnI,exposingapolymerizationsite(17-20)ontheAαchain.TheseregionsbindtocomplimentaryregionsontheDdomainofFbntoformprotofibrils.SubsequentIIacleavageofFPB(R14-G15)fromtheBβchainexposesadditionalpolymerizationsitesandpromoteslateralgrowthoftheFbnnetwork.

Eachofthe2domainsbetweenthecentralnode(Edomain)andtheC-terminalnodes(Ddomain)iscomposedofparallelα-helicalregionsoftheAα,Bβandγchainscoiledaroundeachothertoforma"coiledcoil"withpolarresiduesdirectedoutwardandnonpolarresiduesformingahydrophobiccore.Inthisregion,all3chainspossessaprotease(plasmin)sensitivesite.Theothermajorplasminsensitivesiteisinthehydrophilicpreturbanceoftheα-chainfromtheC-terminalnode.ControlledplasmindegradationatthesesitesconvertsFbgintofragmentDandfragmentE.Theindividualfragmentsareisolatedbysaltfractionation,gelfiltrationandionexchangechromatography.Thefragmentsaresuppliedlyophilizedforstorageat4°C.Highlypurifiedresearchgradefibrinogen(>95%clottable)ispreparedbyacombinationofconventionalandaffinitytechniques.Itissuppliedasafrozensolutioninctirate-phosphateforstorageat-80°C.

Properties:

LocalizationPlasma,platelets
Plasmaconcentration2.6mg/ml
ModeofactionPrecursormoleculewhichiscleavedbythrombintoformfibrinclot.
Molecularweight340,000
Extinctioncoefficient
E
1%
1cm,280nm
=15.1
Isoelectricpoint5.1-6.3
StructureDimerof3pairsofnon-identicalchainsAα(Mr=66,800),Bβ(Mr=52,000)andγ(Mr=46,500),Elongatedtrinodularmoleculewith2terminalDdomainsandonecentralEdomain.FactorXIIIacross-linkingsitesatE328,E366,K508,K584inAαchain;atE398andK406inBβchain.
Percentcarbohydrate3%
Post-translationalmodificationsAαchain:2phosphorylatedserines,S3,S346
Bβchain:N364glycosylationsiteMr=2500,N-terminalpyroglutamicacid
γchain:glycosylatedN52
品牌介绍

Haemtech生物制药服务(HBS)是一家领先的,符合cGMP要求的QC测试实验室,专门为生产重组和血浆衍生蛋白疗法的生物制药制造商提供分析服务,重点是那些会影响止血系统的药物。HBS使客户能够满足法规和质量测试要求,从而将其候选药物从开发转向商业化。

对生物制药的需求正在增长,并且该行业正在迅速采用外包策略,以作为满足不断升级的监管要求的一种方式。凭借在止血方面的丰富经验,HBS能够很好地协助开发影响凝血系统的药物产品。


自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔