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主营:血浆蛋白
℡ 4000-520-616
℡ 4000-520-616
Haematologic Technologies/Bovine Lactadherin/BLAC-1200/50 µg
产品编号:BLAC-1200
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 蛋白类>天然蛋白>球蛋白>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: haemtech
公      司:Haematologic Technologies Inc.
公司分类:
Haematologic Technologies/Bovine Lactadherin/BLAC-1200/50 µg
商品介绍
Formulation70mMSodiumPhosphate,pH7.0
Storage-80°C
Purity>95%bySDS-PAGE
ActivityDeterminationN/A
ShelfLife(properlystored)12months
TheStructureofLactadherin
Lactadherin(MFG-E8)binds,Ca++-independently,tophosphatidyl-L-serineviatheC2domainandbindstheavb3andavb5integrinsviathesecondEGFdomain.Theoneletteraminoacidcodesrefertoidentifiedaminoacidsthatparticipateinbinding.FortheC2domainthecapitallettersrefertowater-exposedhydrophobicresidues.

SampleGelInformation:

GelNovex4-12%Bis-Tris
LoadBovineLactadherin,1µgperlane
BufferMOPS
StandardSeeBluePlus2;Myosin(191kDa),PhosphorylaseB(97kDa),BSA(64kDa),GlutamicDehydrogenase(51kDa),AlcoholDehydrogenase(39kDa),CarbonicAnhydrase(28kDa),MyoglobinRed(19kDa),Lysozyme(14kDa)

Overview:

Lactadherinisawidelydistributedglycoprotein(~50kDa),whichwasoriginallycharacterizedduetoitsassociationwithmilkfat/lipidglobulemembranes.SynonymousnamesarePAS-6/7,bovine-associatedmucoprotein,BA-46,P47,andMFG-E8.Structuralhallmarksoflactadherinarethepresenceoftwoepidermalgrowthfactor(EGF)homologydomains(withanRGDpeptidemotifinthesecondEGFdomain),andtwoCdomainssharinghomologywiththediscoidinfamilyoflectindomainsincludingthephospholipid-bindingdomainsofbloodclottingfactorsVandVIII.Lactadherinshowspreferentialbindingtophosphatidylserine(L-form)inacalciumindependentmanner,andbindsmorespecificallythanAnnexinV.

Purifiedlactadherinfunctionsasananticoagulantbyblockingphosphatidylserine-containingmembranesitesforbloodcoagulationproteins(10).Fluoresence-labeledlactadherinfunctionsasasensitiveprobeforexposedphosphatidylserineonnucleatedcellsandonstimulatedplatelets(8,9).LactadherinwillbindtomembranesthathavephosphatidylserinecontentbelowthethresholdforannexinVbinding.

Lactadherinispurifiedfromun-pasteurizedbovinemilk(11).

IllustratedApplications

K562 cells stained with lactadherin and annexin V

HL60 cellsstained with lactadherin and annexin V  

Above:K562cells(top)andHL60cells(bottom)co-stainedwithbothFITC-conjugatedlactadherin(green)andAlexa-647conjugatedannexinV(red)earlyinapoptosis.Theannexinisinternalizedingranulesandisnotdetectablystainingthecells. Reference:Shi,J.,Y.Shi,L.N.Waehrens,J.T.Rasmussen,C.W.HeegaardandG.E.Gilbert(2006)."Lactadherindetectsearlyphosphatidylserineexposureonimmortalizedleukemiacellsundergoingprogrammedcelldeath."CytometryA69(12):1193-201. Copyright2006. JohnWiley&Sons,Inc. ReprintedwithpermissionofJohnWiley&Sons,Inc.

HeLa cells stained with Lactadherin after treatment with staurosporine 2 hour

HeLa cells stained with Lactadherin after treatment with staurosporine 3 hour

Above:HeLacellsstainedwithFITC-conjugatedlactadherin2hours(top)and3hours(bottom)aftertreatmentwithstaurosporine.Earlyinapoptosisthecellshavesmallvesiclesandlong,thinappendagesthatstainavidlyforlactadherin.Reference:WaehrensLN,Heeghaard,CW,GilbertGE,RasmussenJT.BovineLactadherinasaCalcium-independentImagingAgentofPhosphatidylserineExpressedontheSurfaceofApoptoticHeLaCells2009J.Histochem.Cytochem.(ePubJune2009).

Mesenteric-venous-thrombosis

Above:Phosphatidylserineexposureinmousemesentericvenousthrombosis. Miceweregiven1µgeachoflactadherinandannexinVbytailveinimmediatelypriortoexternalizationofthemesentery.Themesentarywasexposedtoferricchlorideandthentheanimalswereperfusedwithsaline/paraformaldehyde.Serialsectionsofthemesentarywerestainedwithanti-fibrinogen/fibrin(left),anti-platelet(middle),andanti-lactadherinantibodies(right)developedwiththealkalinephosphataseVectorRedsubstrate.Alayeroffibrinogen/fibrin(left,closedarrows)overlaidamuralhemorrhage(openstar).Platelets(middle)werescatteredalongtheluminalsurfaceofthethrombus(opentriangles)aswellasuponfibrinogen/fibrinstrandsextendingintothelumen.Lactadherinstaining(right)wasstrongestalongtheraisedendotheliumsurface(closedarrow),includingadherentplateletsclosetothewall.Plateletsonfibrinstrandsdidnotstaindetectably(openarrow).(ShiJ,PipeSW,RasmussenJT,HeegaardCW,GilbertGE.Lactadherinblocksthrombosisandhemostasisinvivo:correlationwithplateletphosphatidylserineexposure.JThrombHaemost.Jul2008;6(7):1167-1174).

Properties:

NamesLactadherin,MFG-E8,PAS-6/7,SED1,p47
LocalizationMilk(1),alsosecretedbystimulatedmacrophages(2),vascularsmoothmuscle(3),endothelialcells(4)
Plasmaconcentrationunknowninnormaladults,measurableinwomenwithmetastaticbreastcancer(5),measurableinnewborncalvesaftermilkmeal(1)
ModeofactionPhysiologicfunctionasopsoninorbridgeligand(6,7).Thelectin-likedomainsengagephosphatidyl-L-serine,thesecondEGFdomainengagesavb3and/oravb5integrinleADIngtophagocytosisofapoptoticcells.
Molecularweight52kDaand47kDa(glycosylationvariants,PAS-6andPAS-7respectively)
Extinctioncoefficient
E
1%
1cm,280nm
=16.5(calculated)
StructuresinglechainwithtwoEGFdomainsandtwoCdomains.ThemouseisoformcontainsacentralprolinerichregionandthehumanmoleculecontainsasingleEGFdomain
Percentcarbohydrate6%,13%forglycosylationvariants
Post-translationalmodificationsglycosylation
品牌介绍

Haemtech生物制药服务(HBS)是一家领先的,符合cGMP要求的QC测试实验室,专门为生产重组和血浆衍生蛋白疗法的生物制药制造商提供分析服务,重点是那些会影响止血系统的药物。HBS使客户能够满足法规和质量测试要求,从而将其候选药物从开发转向商业化。

对生物制药的需求正在增长,并且该行业正在迅速采用外包策略,以作为满足不断升级的监管要求的一种方式。凭借在止血方面的丰富经验,HBS能够很好地协助开发影响凝血系统的药物产品。


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