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主营:血浆蛋白
℡ 4000-520-616
℡ 4000-520-616
Haematologic Technologies/CTI vacuum collection tube/SCAT-27/25 X 2 mL, 25 X 3 mL, 25 X 5 mL
产品编号:SCAT-27
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 蛋白类>天然蛋白>球蛋白>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: haemtech
公      司:Haematologic Technologies Inc.
公司分类:
Haematologic Technologies/CTI vacuum collection tube/SCAT-27/25 X 2 mL, 25 X 3 mL, 25 X 5 mL
商品介绍
Formulation11mMCitrate,50ug/mLCTI(final)
MolecularWeight
Storage4°C
PurityN/A
Compound
AssayN/A
ShelfLife(properlystored)6months
ChemicalFormula
EffectofCTIontheAPTTandPTofNormalHumanPlasma
ThegraphillustratestheeffectofaddedCTIonthePTandaPTTofnormalhumanplasma.CTIwasaddedtonormalpooledhumanplasmatovariousfinalconcentrations,andthemixtureswereallowedtoincubateforabriefperiodonicepriortoconductingtheassays.ThePTremainsunaffectedwhiletheaPTTdoubleswiththeadditionofapproximately60microgramsofCTIpermlofplasma.

SampleGelInformation:

GelNovex4-12%Bis-Tris
LoadCornTrypsinInhibitor,1µgperlane
BufferMES
StandardSeeBluePlus2;Myosin(188kDa),PhosphorylaseB(98kDa),BSA(62kDa),GlutamicDehydrogenase(49kDa),AlcoholDehydrogenase(38kDa),CarbonicAnhydrase(28kDa),MyoglobinRed(17kDa),Lysozyme(14kDa),Aprotinin(6kDa),Insulin,Bchain(3kDa).

Overview:

PurifiedCTI
Corntrypsininhibitor(CTI)isasmallproteinthatislocalizedinthekernelsofmostspeciesofcorn.CTIisnotonlyaninhibitoroftrypsin,butisalsoaspecifichumanfactorXIIainhibitorwhentestedinbloodclottingexperiments(1-6).Theinhibitorformsaone-to-onecomplexwitheithertrypsinorfactorXIIa,andwhenaddedtoplasma,prolongstheactivatedpartialthromboplastintimewithoutaffectingthePTassay(6).ThespecificityforfactorXIIamakestheinhibitorusefulforthesegregationandstudyoftissuefactor(TF)dependentcoagulationreactions(6).

TheuseofCTItostudyTF-dependentreactionshasbeendocumentedinliterature(6,8-10).StudiesindicatethatsuppressionofthecontactpathwayofcoagulationisessentialwhenattemptingtoperformTF-dependentassaysinwholebloodorplasmasamples.TheadditionofCTIatthepointofsamplecollectionpreventsactivationofthecontactpathwayduringsubsequentsampleprocessingsteps,thusreducingin-vitroartifact.ThemostcommonuseofCTIisassociatedwiththrombingenerationassayswhenattemptingtoworkatlowTFconcentrations.HTI’sCTIproductisperformancetestedforthisspecificapplication.

Theproteiniscomprisedof112aminoacidswhichyieldsacalculatedmolecularweightof12,028(7).Thecalculatedmolecularweightandthatwhichhasbeendeterminedbysedimentationequilibriumanalysesofthepurifiedprotein(12,500)areingoodagreement(3).AcomparisonoftheaminoacidsequenceofCTItothatofothertrypsinorserineproteaseinhibitorsrevealslittleornosimilarities(7).

CTIispurifiedusingacombinationofpublishedprocedures(3,4).Tobeginthepurificationprocess,CTIisextractedfromthekernelsoffreshsweetcornintoaphysiologicbuffer.Theextractisthende-fattedusingacetone,andtheproteinisfurtherpurifiedbyemployinggelfiltrationandion-exchangechromatography.ThefinalCTIpreparationappearsasasinglebandbySDS-PAGEanalysesunderbothreducingandnon-reducingconditions.

PreparationsofCTIaretestedfortheABIlitytoprolongtheaPTTassaywithoutaffectingthePTassay.ThespecificactivityofeachlotofCTIisdetermined,andoneunitisdefinedastheamountofCTIrequiredtodoubletheaPTTofnormalhumanplasma.

PurifiedCTIisformulatedin20mMTris,0.15MNaCl,pH7.4andshouldbestoredfrozenat-20°Corcolder.

BloodCollectionTubes
TosimplifytheprocessofcollectingsampleswithaddedCTI,HTIhasdevelopedbloodcollectiontubescontainingCTI.Thesetubes(ourSCAT-27line)simplifytheprocessofconductingTF-dependentstudiesbyallowingyoutodrawblooddirectlyontoananticoagulantcontainingCTI.YoumaychoosetouseourstandardCTI/Citrateformulation(11mMCitrate,50ug/mLCTI)oryoumaycreateyourowncustomformulation.HTI’sbloodcollectiontubesarenotsterileandaremanufacturedandsoldforresearchuseonly.Threestandardsizesareavailablealthoughcustomsizescanbemanufacturedforyou.

SCAT-27-4.5/5-5mLtube

SCAT-27-2.7/5-3mLtube

SCAT-27-1.8/5-2mLtube

 

Properties:

LocalizationCornKernals
Modeofactionformsaone-to-onecomplexwitheithertrypsinorfactorXIIaandinhibitstheirrespectivecatalyticactivity.
Molecularweight12,500(3)
Extinctioncoefficient
E
1%
1cm,280nm
=20.0(determinedbyultracentrifugationstudies)
Structuresinglechainproteincomprisedof112aminoacids(3,7)
品牌介绍

Haemtech生物制药服务(HBS)是一家领先的,符合cGMP要求的QC测试实验室,专门为生产重组和血浆衍生蛋白疗法的生物制药制造商提供分析服务,重点是那些会影响止血系统的药物。HBS使客户能够满足法规和质量测试要求,从而将其候选药物从开发转向商业化。

对生物制药的需求正在增长,并且该行业正在迅速采用外包策略,以作为满足不断升级的监管要求的一种方式。凭借在止血方面的丰富经验,HBS能够很好地协助开发影响凝血系统的药物产品。


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